| 质粒名称: | pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 48295 |
| 存储实验室: | Scott Gradia |
|
载体骨架基本信息 |
|
| 载体名称: | pFastBac |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5407 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 昆虫表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | His6（主干上的N终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B) was a gift from Scott Gradia (Addgene plasmid # 48295 ; http://n2t.net/addgene:48295 ; RRID:Addgene_48295) |
pFastBac His6 TEV cloning vector with BioBrick polycistronic
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pFastBac His6 TEV cloning vector with BioBrick polycistronic
r... 5407 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pFastBac His6 TEV cloning vector with BioBrick polycistronic
r...
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5407)
TITLE Series-11: Baculovirus Expression Vectors with Biobrick PolyPromoter
Restriction Sites
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5407)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5407
/organism="synthetic DNA construct"
/mol_type="other DNA"
mobile_element 68..292
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
promoter complement(1062..1090)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
primer_bind 1474..1491
/label=Polyhedrin-F
/note="Polyhedrin promoter, forward primer"
CDS 1611..1628
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 1629..1649
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
polyA_signal 1894..2028
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1931..1950)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1985..2004
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
mobile_element 2073..2238
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
rep_origin 2422..2877
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2509..2528)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2719..2740
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 2904..3008
/gene="bla"
/label=AmpR promoter
CDS 3009..3869
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3227..3246)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4040..4628
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4529..4548
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4782..4799
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(4883..4902)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5043..5065
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(5059..5250)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
ORIGIN
1 gaccagccgc gtaacctggc aaaatcggtt acggttgagt aataaatgga tgccctgcgt
61 aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca
121 ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
181 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
241 gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta aagactatat tcgcggcgtt
301 gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt
361 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
421 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
481 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
541 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
601 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
661 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
721 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
781 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
841 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc
901 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
961 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
1021 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
1081 cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
1141 tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg
1201 tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca
1261 cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg
1321 tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt
1381 tcgcccagga ctctagctat agttctagtg gttggctacg tatactccgg actattaata
1441 gatcatggag ataattagcg gccgcttaat taaaaatgat aaccatctcg caaataaata
1501 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataacta ttccggatta
1561 ttcataccgt cccaccatcg ggcgcggatc ccggtccgat gggttcttct caccatcacc
1621 atcaccatga aaacctgtac ttccaatcca atattggaag tggataacgg atccgaattc
1681 gagcgccgtc gacaagcttg cggcctcatg atctcctagg ggcctcatga tctcctaggg
1741 gccgcactcg aggcatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc
1801 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
1861 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
1921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
1981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
2041 gagcctagcg atcgccctgc aggggagatc cgaaccagat aagtgaaatc tagttccaaa
2101 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
2161 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
2221 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc
2281 tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa
2341 atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt
2401 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
2461 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
2521 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
2581 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
2641 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
2701 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
2761 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
2821 agctgattta acaaaaattt aacgcgaatt ttaacaaact attaacgttt acaatttcag
2881 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
2941 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaatac tattgaaaaa
3001 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
3061 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
3121 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
3181 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
3241 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
3301 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
3361 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
3421 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
3481 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
3541 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
3601 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
3661 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
3721 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
3781 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
3841 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
3901 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
3961 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
4021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
4081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
4141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
4201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
4261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
4321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
4381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
4441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
4501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
4561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
4621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
4681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
4741 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
4801 aagcggaaga ggcgcgccat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
4861 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
4921 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
4981 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
5041 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
5101 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
5161 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
5221 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
5281 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
5341 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
5401 ggaccag
//
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